Glucose and Insulin Tolerance Assays

GTT and ITT were performed to respectively detect the ability to regulate blood glucose and insulin sensitivity [32 (link),38 (link)]. In GTT, the mice were fasted for 12 h and then injected with glucose in the dose of 2 g glucose per Kg mice (glucose was purchased from Beyotime Biotech Inc., Shanghai, China, Cat#ST1228). In ITT, mice were fasted for 4 h and then injected with insulin in the dose of 0.75 U insulin per Kg mice (insulin was purchased from Beyotime Biotech Inc., Shanghai, China, Cat#P3376). All experiments were required to detect and record the blood glucose in time points of 0, 30, 60, 90, 120, and 150 min after the injection. Blood glucose was measured from the second drop of tail blood using a Roche blood glucose meter (Roche Pharma (Schweiz) Ltd., Basel, Switzerland).