Histological Evaluation of Osteoarthritic Cartilage

Whole knee joints (n = 5) were fixed in 4% paraformaldehyde, decalcified, paraffin-embedded, and sectioned at 6 μm thickness for histological and immunohistochemical evaluation. The sections were stained with saffron O (Solarbio, Beijing, China), hematoxylin-eosin (Solarbio), or saffron O/solid green (Solarbio) to reveal histological changes in the cartilage. Cartilage destruction severity was graded according to the modified Mankin [40 (link)] and Osteoarthritis Research Society International (OARSI) [41 (link)] scoring systems (scoring criteria are provided in Tables S2 and S3).

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Jin X., Dong X., Sun Y., Liu Z., Liu L, & Gu H. (2022). Dietary Fatty Acid Regulation of the NLRP3 Inflammasome via the TLR4/NF-κB Signaling Pathway Affects Chondrocyte Pyroptosis. Oxidative Medicine and Cellular Longevity, 2022, 3711371.

Publication 2022

hematoxylin-eosin

Cartilage Eosin Hematoxylin Knee joints Osteoarthritis Paraffin Paraformaldehyde Saffron

Corresponding Organization : China Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cartilage destruction severity

control variables

Whole knee joints (n = 5)
Fixation in 4% paraformaldehyde
Decalcification
Paraffin embedding
Sectioning at 6 μm thickness

controls

Positive control: Not mentioned
Negative control: Not mentioned

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