Immunohistochemical Staining and Imaging Protocol

Tissue specimens were processed and stained as previously described^{1 (link)}. Slides were stained with primary antibodies (Phospho-Paxillin (Tyr118), 1:100, Cell Signaling; ACP6, 1:50, Abcam; p53 (Pantropic), 1:100, Millipore Sigma; Ki-67, 1:200, Thermo Fisher Scientific) in 1.25% normal horse serum/PBS or 20% goat serum/TBST overnight at 4 °C. For IHC, slides were visualized using VECTASTAIN Elite ABC HRP kit and DAB Substrate Kit (Vector Laboratories) and counterstained with hematoxylin (Thermo Fisher Scientific). For IF, slides were probed with fluorescent secondary antibodies (1:200, Thermo Fisher Scientific) and Hoechst 33258 (1:200, Thermo Fisher Scientific). Images were acquired with a Nikon Eclipse Ti2 microscope and images processed and quantified with Fiji-ImageJ or NIS-Elements HC.