In Vivo Retinal Imaging of Mouse Models

In vivo retinal imaging was performed as previously described in detail by DeRamus et al. [15 (link)], using a Bioptigen Model 840 Envisu Class-R high-resolution SD-OCT instrument (Bioptigen/Leica, Inc.; Durham, NC, USA). Data were collected from Dhdds^K42E/K42E and WT mice at postnatal day (PN) 1 (KI, n = 5; WT, n = 9), 2 (KI, n = 4; WT, n = 8), 3 (KI, n = 5; WT n = 3), 8 (KI, n = 4; WT n = 5), and 12 months (mos) (KI, n = 3; WT n = 3) to assess retinal structure. Layer thicknesses were determined manually using Bioptigen InVivoVue^® and Bioptigen Diver^® V. 3.4.4 software and the data were analyzed and graphed using Microsoft Excel software.