Phylogenetic Analysis and 3D Docking of BmDXR

The amino acid sequence of BmDXR was aligned with the selected amino acid sequences from other organisms by MAFFT online¹, then edited by BioEdit v7.25, and phylogenetically analyzed by using the Maximum Likelihood method in MEGA 7 (Kumar et al., 2016 (link)). The structure of BmDXR was predicted by SWISS-MODEL² (Guex et al., 2009 (link); Bienert et al., 2017 (link); Waterhouse et al., 2018 (link)). The 3D structure of BmDXR was virtually docked with FSM through Molecular Operating Environment (MOE) version 2014.09 (Chemical Computing Group).

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Wang S., Li M., Luo X., Yu L., Nie Z., Liu Q., An X., Ao Y., Liu Q., Chen J., Tian Y., Zhao J, & He L. (2020). Inhibitory Effects of Fosmidomycin Against Babesia microti in vitro. Frontiers in Cell and Developmental Biology, 8, 247.

Publication 2020

MOE) version 2014.09

Amino acid sequences

Corresponding Organization : Huazhong Agricultural University

Other organizations : National Institute for Parasitic Diseases

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Variable analysis

independent variables

Alignment of BmDXR amino acid sequence with selected amino acid sequences from other organisms using MAFFT online
Phylogenetic analysis of the aligned sequences using the Maximum Likelihood method in MEGA 7
Prediction of the structure of BmDXR using SWISS-MODEL
Molecular docking of BmDXR with FSM through Molecular Operating Environment (MOE) version 2014.09

dependent variables

Inferred phylogenetic relationships between BmDXR and other organisms based on the Maximum Likelihood analysis
Predicted 3D structure of BmDXR
Docking interactions between BmDXR and FSM

control variables

BioEdit v7.25 used to edit the aligned amino acid sequences

positive controls

Not specified

negative controls

Not specified

Annotations

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