Protocol detail

Cigarette Smoke and COPD Exacerbation in A549 Cells

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A549 cells, pulmonary adenocarcinoma derived cell line [18 (link)], purchased directly from Sigma- Aldrich (catalog number 86012804) and cultured in Dulbecco’s modified eagle’s medium (DMEM), (Biological Industries, Beit Haemek, Israel) with 10% fetal calf serum (FCS), 100 units/ml penicillin, and 100 mg/ml streptomycin, under a humidified atmosphere (5% CO2 plus 95% air) at 37°C. Treatments included 2%, 4% and 10% CSE. LPS was added to the cultures to stimulate COPD exacerbation condition [6 (link)].

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Nachmias N., Langier S., Brzezinski R.Y., Siterman M., Stark M., Etkin S., Avriel A., Schwarz Y., Shenhar-Tsarfaty S, & Bar-Shai A. (2019). NLRP3 inflammasome activity is upregulated in an in-vitro model of COPD exacerbation. PLoS ONE, 14(5), e0214622.

Publication 2019

A549 cells DMEM

A549 cells Adenocarcinoma Atmosphere Biological Cell line Copd Cultured medium Eagle Fetal calf serum Penicillin Pulmonary Streptomycin

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Variable analysis

independent variables

2% CSE
4% CSE
10% CSE

dependent variables

COPD exacerbation condition

control variables

Dulbecco's modified eagle's medium (DMEM)
10% fetal calf serum (FCS)
100 units/ml penicillin
100 mg/ml streptomycin
Humidified atmosphere (5% CO2 plus 95% air) at 37°C

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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