Integrated Multi-Omics Analysis of Dorsolateral Prefrontal Cortex

The discovery PWAS data were obtained from the dorsolateral prefrontal cortex (dPFC) of postmortem brain tissues from 376 subjects recruited by the Religious Orders Study/Memory and Aging Project (ROS/MAP) [19 (link)]. For proteome sequencing, isobaric tandem mass tag peptide labeling was utilized, and peptides were assessed using liquid chromatography coupled to mass spectrometry (MS) [20 (link)]. Wingo et al. [21 (link)] used Thermo Fisher Scientific’s Proteome Discoverer suite v.2.3 and tandem MS spectra to search against the standard UniProtKB human proteome database, which has 20,338 total sequences, to assign peptide spectral matches. Genotyping was done using whole-genome sequencing or genome-wide genotyping on the Illumina OmniQuad Express or Affymetrix GeneChip 6.0 platforms. Over 8356 proteins having both proteomic and genomic data, among which 1475 protein could find significant cis associations with genetic variation.