Characterization of HGSOC Cell Lines

The four human HGSOC cell lines used, OV1369(R2), OV90, OV4453, and OV1946, were derived in our laboratory from the ascites of patients diagnosed with HGSOC and have been extensively characterized^{23 (link)–26 (link)}. All cell lines were maintained in a low oxygen condition (7% O₂ and 5% CO₂) and grown in OSE medium (Wisent, Montreal, QC) with 10% fetal bovine serum (FBS) (Wisent), 0.5 μg ml⁻¹ amphotericin B (Wisent) and 50 μg ml⁻¹ gentamicin (Life Technologies Inc., Burlington, ON). The MDA-MB-231 breast cancer cell line was a gift from the laboratory of Dr. John Stagg (CRCHUM, Canada) which was purchased from ATCC. It was maintained in DMEM (Wisent) with 10% FBS (Wisent), 0.5 μg ml⁻¹ amphotericin B (Wisent) and 50 μg ml⁻¹ gentamicin (Life Technologies Inc.). All HGSOC cell lines were authenticated in 2017 using short tandem repeat (STR) profiling by the McGill University Genome Center (Montreal, Canada). All cell lines were tested negative for mycoplasma with IDEXX BioAnalytics (Columbia, MO65201).