Immunohistochemical Staining of Neural Markers

The specimens were processed for H& E staining and IHC as described [8 (link)], with antibodies for NHERF1 1:3200 (Thermo/Fisher, Waltham, MA), moesin 1:100 (3150, Cell Signaling Technology, Danvers, MA), Kir7.1 1:200, NF2 C-terminal 1:800 (C18) (see also [19 (link)]) (Santa Cruz Biotechnology, Santa Cruz, CA) and ezrin 1:400 (30252, BD Biosciences, San Jose, CA). Two certified neuropathologists reviewed independently the IHC results. Images were acquired and analyzed at various magnifications with an Aperio Scanscope CS2 whole slide image system (Leica Biosystems, San Diego, CA).

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Georgescu M.M., Mobley B.C., Orr B.A., Shang P., Lehman N.L., Zhu X., O’Neill T.J., Rajaram V., Hatanpaa K.J., Timmons C.F, & Raisanen J.M. (2016). NHERF1/EBP50 and NF2 as diagnostic markers for choroid plexus tumors. Acta Neuropathologica Communications, 4, 55.

Publication 2016

NHERF1 moesin NF2 C-terminal 1:800 ezrin Aperio Scanscope CS2 whole slide image system

Antibodies Ezrin Moesin Neuropathologists Nherf1

Corresponding Organization : The University of Texas Southwestern Medical Center

Other organizations : Vanderbilt University Medical Center, St. Jude Children's Research Hospital, The Ohio State University, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Antibodies used for IHC: NHERF1 1:3200, moesin 1:100, Kir7.1 1:200, NF2 C-terminal 1:800 (C18), ezrin 1:400

dependent variables

IHC results

control variables

H&E staining as described [8 (link)]
Two certified neuropathologists reviewed the IHC results independently
Images were acquired and analyzed at various magnifications with an Aperio Scanscope CS2 whole slide image system

positive controls

Not specified

negative controls

Not specified

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