Human chondrocyte CHON002 cell line (ATCC RL-2847) (5×105 cells) were obtained from Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia. The cells were seeded into T-25 flasks and incubated for 48 hr. The medium (hWJMSCs-CM) was replaced and the cells treated with recombinant IL1β (Biolegend, 579404) with concentrations of 0 (no treatment) and 10 ng/ml for 5 days in preparation for the OA model 20 –22 .
The experiment were conducted with 6 different groups as follow: 1) CHON002 without IL1β induction and without additions of CM (control); 2) IL1β-CHON002 without additions of hWJMSCs-CM; 3) IL1β-CHON002 treated with hWJMSCs-CM 15% (v/v); 4) IL1β-CHON002 treated with hWJMSCs-CM 30% (v/v); 5) IL1β-CHON002 treated with CM of IGF1-hWJMSCs (IGF1-hWJMSCs-CM) 15% (v/v); 6) IL1β-CHON002 treated with IGF1-hWJMSCs-CM 30% (v/v). The medium were replaced every 2 days. The experiment were carried for 1 and 2 weeks 21 (link),23 .
The experiment were conducted with 6 different groups as follow: 1) CHON002 without IL1β induction and without additions of CM (control); 2) IL1β-CHON002 without additions of hWJMSCs-CM; 3) IL1β-CHON002 treated with hWJMSCs-CM 15% (v/v); 4) IL1β-CHON002 treated with hWJMSCs-CM 30% (v/v); 5) IL1β-CHON002 treated with CM of IGF1-hWJMSCs (IGF1-hWJMSCs-CM) 15% (v/v); 6) IL1β-CHON002 treated with IGF1-hWJMSCs-CM 30% (v/v). The medium were replaced every 2 days. The experiment were carried for 1 and 2 weeks 21 (link),23 .
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