Staining was performed using standard immunohistochemical procedures as previously described.[41 (link)
] Sections and primary microglia were washed 3 times with 1 × PBS and blocked with 5% normal goat serum (Gibco) and 0.2% Triton X‐100 for 1 h. Then, sections were incubated with specific antibodies at 4 °C overnight: anti‐Sting (1:200; 19851‐1‐AP, ProteinTech), anti‐Mfn2 (1:200; ab124773, Abcam), anti‐Iba1 (1:200; ab289874, Abcam), and anti‐iNOS (1:200; ab178945, Abcam). 4′,6‐diamidino‐2‐phenylindole (DAPI) (1:1000, Invitrogen) was used to label the nuclei. All sections and microglia were imaged using a Nikon confocal microscope and analyzed using ImageJ software.
] Sections and primary microglia were washed 3 times with 1 × PBS and blocked with 5% normal goat serum (Gibco) and 0.2% Triton X‐100 for 1 h. Then, sections were incubated with specific antibodies at 4 °C overnight: anti‐Sting (1:200; 19851‐1‐AP, ProteinTech), anti‐Mfn2 (1:200; ab124773, Abcam), anti‐Iba1 (1:200; ab289874, Abcam), and anti‐iNOS (1:200; ab178945, Abcam). 4′,6‐diamidino‐2‐phenylindole (DAPI) (1:1000, Invitrogen) was used to label the nuclei. All sections and microglia were imaged using a Nikon confocal microscope and analyzed using ImageJ software.
Free full text: Click here
