Superoxide was measured using dihydroethidium (DHE) and an HPLC-based assay with minor modification as described previously 22 (link). NADPH oxidase activity was measured in membrane preparations prepared as described previously using ESR and the spin probe CPH 23 (link), and was quantified as NADPH dependent O2∸ production. NO• levels in endothelial cells and vessels were quantified by ESR and colloid Fe(DETC)2 as described previously 24 (link).
Protocol detail
Measuring Oxidative Stress and Nitric Oxide
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Assay
Colloid
Dihydroethidium
Endothelial cells
Hplc
Membrane
Nadph
Nadph oxidase
Superoxide
Vessels
Corresponding Organization :
Other organizations : Emory University
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Variable analysis
independent variables
- Superoxide was measured using dihydroethidium (DHE)
- NADPH oxidase activity was measured in membrane preparations
- Superoxide levels
- NADPH dependent O2∸ production
- NO• levels in endothelial cells and vessels
- Membrane preparations were prepared as described previously
- No positive or negative controls were explicitly mentioned.
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