P. aeruginosa isolates were grown in LB broth (Neogen, Lansing, MI, USA) for 24 h at 30 °C. Ten microliters of a P. aeruginosa suspension containing 108 CFU/mL in the presence (1/2, 1/4, and 1/8 MIC) or absence (control) of subinhibitory concentrations of bio-AgNPs were inoculated at the center of swarming agar plates containing 1% w/v glucose (Synth, Diadema, SP, Brazil), 0.5% w/v peptone (Acumedia, Lansing, MI, USA), 0.2% w/v yeast extract (BD, Sparks, MD, USA), and 0.5% w/v agar (BD, Sparks, MD, USA). Plates were incubated without inversion for 24 h at 30 °C [22 (link)].
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