Human SCAP were isolated from an extracted immature mandibular third molar of a male patient aged 18 years old by a method used in a previous study (11 (link)). All protocols were reviewed and approved by the Ethics Committee of Guanghua School and Hospital of Stomatology, Sun Yat-sen University (Guangzhou, China). The osteogenic and adipogenic differentiation capacities of SCAP were identified by Alizarin Red staining and Oil Red O staining (Cyagen Biosciences, Inc., Guangzhou, China), respectively (11 (link)). The typical phenotypes, including STRO-1/Alexa Fluor 647-Allophycocyanin (BioLegend, Inc., San Diego, CA, USA), CD146/Phycoerythrin (BD Pharmingen, San Diego, CA, USA), CD24/Fluorescein Isothiocyanate (FITC) (BD Pharmingen) and CD45/FITC (BD Pharmingen), using the 2nd passage of SCAP were assessed using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Protocol detail
Isolation and Characterization of SCAP
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Alizarin Red staining
Oil Red O
CD146/Phycoerythrin
CD24/Fluorescein Isothiocyanate (FITC)
CD45/FITC
FACSCalibur flow cytometer
Adipogenic
Alexa fluor 647
Allophycocyanin
Ethics committee
Fluorescein
Human
Isothiocyanate
Male
Mandibular
Osteogenic
Patient
Phenotypes
Phycoerythrin
Third molar
Corresponding Organization :
Other organizations : Stomatology Hospital, Sun Yat-sen University
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Variable analysis
independent variables
- Isolation method of human SCAP from an extracted immature mandibular third molar of a male patient aged 18 years old
- Osteogenic differentiation capacity of SCAP
- Adipogenic differentiation capacity of SCAP
- Typical phenotypes of SCAP (STRO-1, CD146, CD24, CD45) at the 2nd passage
- Ethical approval for the study protocols by the Ethics Committee of Guanghua School and Hospital of Stomatology, Sun Yat-sen University (Guangzhou, China)
- Not explicitly mentioned
- Not explicitly mentioned
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