Trifluoroacetic acid, Harris-modified hematoxylin, and α-cyano-4-hydroxycinnamic acid (CHCA) were obtained from Sigma Aldrich (St. Louis, MO). HPLC grade methanol, ethanol, acetonitrile, xylene, hydrogen peroxide and water were obtained from Fisher Scientific (Pittsburgh, PA). Tissue Tack microscope slides were purchased from Polysciences Inc (Warrington, PA). Citraconic anyhydride and SafeClear II was purchased from Thermo Scientific (Bellefonte, PA). Recombinant Peptide N-Glycosidase F (PNGase F) from Flavobacterium menigosepticum was obtained, expressed, and purified as previously described [34 (link)], but is also available commercially as PNGase F Prime™ from Bulldog Bio (Portsmouth, NH). Universal Antigen Retrieval Reagent was purchased from R&D Systems (Minneapolis, MN).
Protocol detail
MALDI-TOF MS Sample Preparation
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Trifluoroacetic acid
Harris-modified hematoxylin
α-cyano-4-hydroxycinnamic acid
HPLC grade methanol
ethanol
acetonitrile
Tissue Tack microscope slides
PNGase F Prime™
Acetonitrile
Antigen
Ethanol
Flavobacterium
Hematoxylin
Hplc
Hydrogen peroxide
Hydroxycinnamic acid
Methanol
Microscope
Peptide n glycosidase f
Tissue
Trifluoroacetic acid
Xylene
Corresponding Organization : Medical University of South Carolina
Other organizations : Drexel University
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Variable analysis
independent variables
- Trifluoroacetic acid
- Harris-modified hematoxylin
- α-cyano-4-hydroxycinnamic acid (CHCA)
- Not explicitly mentioned
- HPLC grade methanol
- HPLC grade ethanol
- HPLC grade acetonitrile
- HPLC grade xylene
- HPLC grade hydrogen peroxide
- HPLC grade water
- Tissue Tack microscope slides
- Citraconic anyhydride
- SafeClear II
- Recombinant Peptide N-Glycosidase F (PNGase F) from Flavobacterium menigosepticum
- Universal Antigen Retrieval Reagent
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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