Colon Cancer Cell Lines Maintenance

CA colon cancer cell lines HT-29 (MSS) and HCT 116 (MSI) were purchased from the American Type Culture Collection (Manassas, VA), while AA colon cancer cell lines, CHTN-06 (MSS) and SB-521 (MSI), were generated at Stony Brook University as previously described (17 (link)). All cell lines were maintained in DMEM media (Corning) supplemented with 10% FBS, 100 U/ml penicillin, 100 U/ml streptomycin, 4.5 g/L glucose and sodium pyruvate, in humidified incubator with 37°C and 5% CO₂. Human recombinant IL-1β was purchased from Cell Signaling Technology and resuspended in PBS for a final stock concentration of 10 ug/ml (catalog #8900). 5-FU was purchased from Sigma-Aldrich (catalog #F6627-1G) and resuspended in DMSO (Thermo Fisher Scientific) for a final stock concentration of 380mM. Human recombinant IL-1Ra was purchased from Sigma-Aldrich (catalog ##SRP3084) and resuspended in PBS supplemented with 0.1% BSA for a final stock concentration of 100 ug/ml.