Investigating TNF-α-Induced Inflammatory Response

The materials are as follows: recombinant TNF-α (PeproTech, NJ, USA); ABT-199, SP600125, and lenalidomide (MedChemExpress, NJ, USA); TNF-α, myeloperoxidase (MPO), interleukin-1 β (IL-1β), IL-6, IL-8, and IL-18 enzyme linked immunosorbent assay (ELISA) kits (BD Biosciences, CA, USA); rabbit anti-mouse FoxO3a antibody, rabbit anti-mouse Ly6G antibody, rabbit anti-mouse JNK antibody, rabbit anti-mouse Bcl-2 antibody, rabbit anti-mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody, horseradish peroxidase- (HRP-) labeled goat anti-rabbit secondary antibody (Abcam, Cambridge, UK); HRP-labeled anti-mouse secondary antibody, bicinchoninic acid (BCA) Protein Assay Kit (Beyotime Biotechnology, Shanghai, China); Naphthol AS-D Chloroacetate (Specific Esterase) Kit (Sigma, NY, USA); Diaminobenzidine DAB Kit (ZLI9019; ZSGB-BIO); Dulbecco's modified Eagle's medium (DMEM) medium, trypsin, and fetal bovine serum (FBS) (Gibco, NY, USA); FoxO3a small interfering ribonucleic acid (siRNA) (Guangzhou RiboBio Co., Ltd., Guangzhou, China); and Cell counting kit-8 (CCK-8) and Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) Apoptosis Assay Kit (Jiangsu KeyGEN BioTECH Corp., Ltd., Jiangsu, China).

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Chen D., Chen C., Xiao X., Huang Z., Huang X, & Yao W. (2021). TNF-α Induces Neutrophil Apoptosis Delay and Promotes Intestinal Ischemia-Reperfusion-Induced Lung Injury through Activating JNK/FoxO3a Pathway. Oxidative Medicine and Cellular Longevity, 2021, 8302831.

Publication 2021

recombinant TNF-α ABT-199 SP600125 lenalidomide TNF-α horseradish peroxidase- (HRP-) labeled goat anti-rabbit secondary antibody BCA) Protein Assay Kit Naphthol AS-D Chloroacetate (Specific Esterase) Kit Diaminobenzidine DAB Kit

Abt 199 Annexin v Anti antibody Apoptosis Assay Bcl 2 Bicinchoninic acid Chloroacetate Enzyme linked immunosorbent assay Esterase Fetal bovine serum Fluorescein Glyceraldehyde 3 phosphate dehydrogenase Goat Il 18 Interleukin 1 Isothiocyanate Lenalidomide Mouse Myeloperoxidase Naphthol Propidium iodide Protein Rabbit Ribonucleic acid Sp600125 Tnf α Trypsin

Corresponding Organization : Third Affiliated Hospital of Sun Yat-sen University

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Variable analysis

independent variables

Recombinant TNF-α
ABT-199
SP600125
Lenalidomide
FoxO3a small interfering ribonucleic acid (siRNA)

dependent variables

TNF-α
Myeloperoxidase (MPO)
Interleukin-1 β (IL-1β)
IL-18
FoxO3a
Bcl-2
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Cell viability
Apoptosis

control variables

Dulbecco's modified Eagle's medium (DMEM) medium
Trypsin
Fetal bovine serum (FBS)

positive controls

Not specified

negative controls

Not specified

Annotations

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