Mouse Bone Marrow FDC-P1 Cell Culture and Irradiation

Mouse bone marrow FDC-P1 cells were purchased from the American Type Culture Collection (ATCC). FDC-P1 cells were cultured in RPMI1640 supplemented with 10% fetal bovine serum (FBS) and 0.5 ng/mL recombinant mouse IL-3 (Peprotech, No.213-13) at 37 °C with 5% CO₂. DIDS sodium salt (MedChemExpress, No.67483-13-0) was reconstituted in sterile water at 500 mM of stock solution and used at 100 μM. DMSO was used to dissolve the powder of RU. 521 (MedChemExpress, No. HY-114180), and the concentration of mother liquor was 20 mM. The solution was frozen at −80 °C and diluted to 10 nM when used. At room temperature, cell cultures were irradiated with cobalt-60 γ-rays with the dose rate of 80.74 cGy/min [53 (link)].