The OPFR mixture was a combination of tris(1,3-dichloro-2-propyl)phosphate (TDCPP, Sigma Aldrich, St. Louis, MO, CAS#:13674-87-8, 95.6% purity), triphenyl phosphate (TPP, Sigma Aldrich, St. Louis, MO; CAS#:115-86-6, 99% purity), and tricresyl phosphate (TCP, AccuStandard, New Haven, CT, CAS#: 1330-78-5, 99% purity) at 1 mg/kg each. This dose was previously found to be effective in an adult exposure model in mice (Krumm et al 2018 (link)), in our recent maternal exposure study on neonatal and juvenile gene expression (Adams et al 2020 (link)), and in a developmental exposure model in rats focusing on exploratory behavior and locomotion (Baldwin et al 2017 (link); Patisaul et al 2013 (link)). To prepare the OPFR stock, each compound was then dissolved into 1 ml of acetone to create a for a stock solution. 100 μl of stock solution was dissolved into 10ml of sesame oil (Sigma-Aldrich) and allowed to vent for 24–48 h, as previously reported (Krumm et al 2018 (link)).
Protocol detail
OPFR Mixture Preparation Protocol
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Acetone
Adult
Gene expression
Locomotion
Maternal exposure
Mice
Neonatal
Phosphate
Rats
Sesame oil
Tricresyl phosphate
Triphenyl phosphate
Tris
Corresponding Organization :
Other organizations : Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- OPFR mixture (1 mg/kg each of TDCPP, TPP, and TCP)
- Neonatal and juvenile gene expression
- Exploratory behavior
- Locomotion
- Dose of OPFR mixture (1 mg/kg each)
- Solvents used (acetone, sesame oil)
- Exposure duration (24-48 hours)
- Positive control: Adult exposure model in mice (Krumm et al. 2018)
- Positive control: Maternal exposure study on neonatal and juvenile gene expression (Adams et al. 2020)
- Positive control: Developmental exposure model in rats focusing on exploratory behavior and locomotion (Baldwin et al. 2017; Patisaul et al. 2013)
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