Levels of circulating cytokines were performed using a Luminex-based Mouse 32-Plex Cytokine Kit (Cat# MCYTMAG-70K-PX32, EMD Millipore, Billerica, MA). Analysis was performed on serum samples that were prepared according to Millipore’s instructions. In brief, peripheral blood was collected and allowed to clot for 30 minutes. Afterwards, blood samples were centrifuged at 1000 x g for 10 minutes. Serum samples were then aliquoted (~25 μL) in to clean tubes and frozen at −80°C until analysis. Before analysis, samples were diluted 1:2 in serum matrix and assayed according to the manufacturer’s instructions. Data were acquired using the FlexMAP 3D system and xPONENT software (Luminex; Austin, TX), and analyzed using MILLIPLEX Analyst software (EMD Millipore) as previously described(27 (link)). Single-plex cytokine-specific ELISAs (R&D Systems), including Cxcl1 (Cat# MKC00B), Cxcl2 (Cat# MM200), Ccl11 (Cat # MME00) and IL-6 (Cat# M6000B), were then used to validate the changes observed in individual cytokines.