Broad-Spectrum Antibiotics in Mice

Mice were exposed to a previously published broad-spectrum regimen of enteric antibiotics as previously described (34 (link)). The following concentrations of antibiotics were administered via drinking water for 3 weeks before oxygen exposure: ampicillin (1 g/liter; Sigma-Aldrich), neomycin sulfate (1 g/liter; Sigma-Aldrich), metronidazole (1 g/liter; Sanofi-Aventis), and vancomycin (0.5 g/liter; Sandoz).

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Ashley S.L., Sjoding M.W., Popova A.P., Cui T.X., Hoostal M.J., Schmidt T.M., Branton W.R., Dieterle M.G., Falkowski N.R., Baker J.M., Hinkle K.J., Konopka K.E., Erb-Downward J.R., Huffnagle G.B, & Dickson R.P. (2020). Lung and gut microbiota are altered by hyperoxia and contribute to oxygen-induced lung injury in mice. Science translational medicine, 12(556), eaau9959.

Publication 2020

ampicillin neomycin sulfate metronidazole vancomycin

Ampicillin Antibiotics Metronidazole Mice Neomycin sulfate Oxygen Regimen Vancomycin

Corresponding Organization : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Exposure to a previously published broad-spectrum regimen of enteric antibiotics

dependent variables

Oxygen exposure

control variables

Concentrations of antibiotics administered via drinking water for 3 weeks before oxygen exposure: ampicillin (1 g/liter), neomycin sulfate (1 g/liter), metronidazole (1 g/liter), and vancomycin (0.5 g/liter)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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