Cell Traction Force Quantification

We followed the protocols of cell-traction quantification published previously^{11 (link)}. In brief, cells were plated on polyacrylamide gels with red fluorescent beads (0.2 μm in diameter; Molecular Probes®, Invitrogen) embedded on the top surface.. Before and after cell trypsinization, fluorescent images were taken to compute the displacement field of the beads using digital image correlation (DIC)in a homebuilt MATLAB program. Cell tractions were then calculated from the displacement field using inverse Boussinesq mathematical model.

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Tan Y., Tajik A., Chen J., Jia Q., Chowdhury F., Wang L., Chen J., Zhang S., Hong Y., Yi H., Wu D.C., Zhang Y., Wei F., Poh Y.C., Seong J., Singh R., Lin L.J., Doğanay S., Li Y., Jia H., Ha T., Wang Y., Huang B, & Wang N. (2014). Matrix softness regulates plasticity of tumour-repopulating cells via H3K9 demethylation and Sox2 expression. Nature Communications, 5, 4619.

Publication 2014

red fluorescent beads

Cell Molecular probes Polyacrylamide gels Tractions

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, University of Illinois Urbana-Champaign, La Jolla Bioengineering Institute, University of California, San Diego

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Variable analysis

independent variables

Cell plating on polyacrylamide gels

dependent variables

Displacement field of fluorescent beads
Cell tractions calculated from the displacement field

control variables

Polyacrylamide gels with red fluorescent beads (0.2 μm in diameter) embedded on the top surface
Fluorescent images taken before and after cell trypsinization

controls

No positive or negative controls were explicitly mentioned in the provided information.

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