Dual-Luciferase Reporter Assay System

Firefly (Photinus pyralis) and Renilla reniformis luciferase activities were assessed using the Dual-Luciferase Reporter Assay System (Promega) for transactivation assays, following the company protocol. The firefly luciferase activity was normalized on Renilla luciferase activity using the Lumat LB 9507 Tube Luminomiter (Berthold).

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Kunkowska A.B., Fontana F., Betti F., Soeur R., Beckers G.J., Meyer C., De Jaeger G., Weits D.A., Loreti E, & Perata P. (2023). Target of rapamycin signaling couples energy to oxygen sensing to modulate hypoxic gene expression in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 120(3), e2212474120.

Publication 2023

Assays Firefly Firefly luciferase Luciferase Photinus Promega Renilla luciferase Transactivation

Corresponding Organization :

Other organizations : Scuola Superiore Sant'Anna, Westfälische Hochschule, Institut Jean-Pierre Bourgin, AgroParisTech, Université Paris-Saclay, Vlaams Instituut voor Biotechnologie, Utrecht University, National Research Council, Institute of Agricultural Biology and Biotechnology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Firefly luciferase activity
Renilla luciferase activity

control variables

The firefly luciferase activity was normalized on Renilla luciferase activity using the Lumat LB 9507 Tube Luminomiter (Berthold)

controls

Positive control: Renilla luciferase
Negative control: Not explicitly mentioned

Annotations

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