Monoclonal Antibodies for HTNV Detection

Monoclonal antibodies (mAb) 1A8 (specific to the HTNV NP; Xu et al., 2002 (link)), Gn-4 (specific to the HTNV Gn), Gc-10 (specific to the HTNV Gc), and 3G1 (with high neutralizing Ab activity against HTNV) were prepared in our laboratory. Our laboratory also supplied the mAb Sp2/0 (Cheng et al., 2014 (link); negative control). Rat anti-mouse GM-CSF and rabbit anti-mouse CD40L antibodies were purchased from Abcam (Cambridge, MA, USA). Other secondary antibodies, including FITC-conjugated antibodies, Cy3-conjugated antibodies, HRP-conjugated antibodies, and PE-conjugated antibodies were purchased from eBioscience (San Diego, CA, USA).