All mice were housed and treated in accordance with NIH and Institutional Animal Care and Use Committee (IACUC) guidelines, and used protocols approved by the Icahn School of Medicine at Mount Sinai or the Gwangju Institute of Science and Technology Animal Care and Use Committees. Studies were conducted in male C57BL/6J mice aged 8–10 weeks (weight, 25 ~ 30 g) purchased from Jackson Laboratories. Cardiac-specific Sirt1 knockout (SIRT1−/−) mice were generated by crossing Sirt1flox/flox mice (Jackson Laboratory) with α-MHC-MerCreMer mice (αMHC-MerCreMer, Jackson Laboratory)19 (link). Conditional cardiomyocyte-specific Serca2 knockout mouse model has been previously described14 . All animal experiments were described in the Supplemental material .
Protocol detail
Cardiac-Specific SIRT1 Knockout Mouse Model
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Animal
C57bl mice
Cardiac
Cardiomyocyte
Institutional animal care and use committee
Knockout mouse
Male
Mice
Serca2
Sirt1
Corresponding Organization :
Other organizations : Icahn School of Medicine at Mount Sinai, Gwangju Institute of Science and Technology, Chonnam National University Hwasun Hospital, University of Shizuoka, Kyoto Medical Center, Rutgers, The State University of New Jersey, KU Leuven
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Variable analysis
independent variables
- Cardiac-specific Sirt1 knockout (SIRT1-/- mice)
- Cardiac function and phenotype
- Male C57BL/6J mice aged 8–10 weeks (weight, 25 ~ 30 g)
- Sirt1flox/flox mice (controls for Sirt1 knockout mice)
- Positive control: Sirt1flox/flox mice
- Negative control: Not explicitly mentioned
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