Zebrafish larvae were imaged in a 384-well imaging system as described previously (Thorn et al., 2019 (link)). Briefly, four 96-well ProxiPlates were placed on a glass stage inside a temperature-controlled cabinet, set at 28.5°C. The upper shelf of the cabinet holds a high-resolution camera (18-megapixel Canon EOS Rebel T6 with an EF-S 55–250 mm f/4.0–5.6 IS zoom lens), connected to a continuous power supply (Canon ACK-E10 AC Adapter) and controlled by a laptop computer. Images are acquired using Canon’s Remote Capture software (EOS Utility, version 3), which is included with the camera. Every 6 seconds, the camera acquires a high-resolution image of larvae in the microplates (Fig 2). Two small speakers (OfficeTec USB Computer Speakers Compact 2.0 System) are located speaker-side down on the glass stage. Speakers were connected by USB to the laptop computer and set to maximum volume (85 dBA). Below the glass plate is a M5 LED pico projector (Aaxa Technologies) with a 900 lumens LED light source, which displays Microsoft PowerPoint presentations to the larvae using the white opaque bottom of the microplates as a back-illuminated screen.