Expansion of NK Cells from PBMCs

PM21 particles were generated and NK cells were expanded from peripheral blood mononuclear cells (PBMCs) as described previously.23 24 (link) Briefly, PBMCs were depleted of T-cells (EasySep CD3 positive selection kit; STEMCELL Technologies) and then cultured for up to 25 days with 100 U/mL interleukin-2 (IL-2; PeproTech) and 200 µg/mL of PM21 particles in SCGM media (CellGenix) supplemented with 10% non-HI FBS. PM21 particles were derived from CSTX-002 (K562-nmIL21-41BBL) cells, provided by Kiadis Pharma and maintained in RPMI media supplemented with 10% FBS.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Varudkar N., Oyer J.L., Copik A, & Parks G.D. (2021). Oncolytic parainfluenza virus combines with NK cells to mediate killing of infected and non-infected lung cancer cells within 3D spheroids: role of type I and type III interferon signaling. Journal for Immunotherapy of Cancer, 9(6), e002373.

Publication 2021

IL-2 SCGM media

Cells Nk cells Peripheral blood mononuclear cells Stemcell T cells

Corresponding Organization : University of Central Florida

Top 5 similar protocols

Variable analysis

independent variables

PM21 particles
Interleukin-2 (IL-2) concentration (100 U/mL)
Duration of PBMC culture (up to 25 days)

dependent variables

Expansion of NK cells from PBMCs

control variables

SCGM media with 10% non-HI FBS
RPMI media with 10% FBS for maintenance of CSTX-002 (K562-nmIL21-41BBL) cells

positive controls

None specified

negative controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!