Imaging FLT3-ITD Signaling in U2OS Cells

U2OS cells transfected with Flag-FLT3-ITD, Flag-FLT3, p27 or p27-Y88F were analyzed as described.^{13 (link)} Briefly, cells were fixed with 4% para-formaldehyde, permeabilized with 0.025% saponine, and blocked with 0.5% gelatine. A Leica DMi8 inverted widefield microscope was used for imaging.

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Peschel I., Podmirseg S.R., Taschler M., Duyster J., Götze K.S., Sill H., Nachbaur D., Jäkel H, & Hengst L. (2017). FLT3 and FLT3-ITD phosphorylate and inactivate the cyclin-dependent kinase inhibitor p27Kip1 in acute myeloid leukemia. Haematologica, 102(8), 1378-1389.

Publication 2017

DMi8 inverted widefield microscope

Cells Flt3 Formaldehyde Gelatine Microscope

Corresponding Organization :

Other organizations : Innsbruck Medical University, Universität Innsbruck, University Medical Center Freiburg, Klinikum rechts der Isar, Technical University of Munich, Medical University of Graz

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Variable analysis

independent variables

Flag-FLT3-ITD
Flag-FLT3
P27-Y88F

dependent variables

Not explicitly mentioned

control variables

Cell line: U2OS

controls

Positive control: Not mentioned
Negative control: Not mentioned

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