Peptide-specific Antibody Enrichment

Ova-conjugated peptides 20, 26 or ovalbumin were immobilized to agarose (Amino-Link, ThermoFisher) and then incubated with pooled human sera that has been previously described [48 (link)]. The bound antibodies were eluted in 0.1M glycine-pH 2.2 and quickly neutralized with 1/10^th volume of 0.1M Tris-pH 9.0 and then buffer exchanged into Tris-Saline pH-7.2 using Zeba buffer exchange columns (ThermoFisher). Antibodies were quantified by SDS-PAGE followed by coomassie staining.

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Chin A., Liu J., Jardetzky T., Johnson D.C, & Vanarsdall A. (2022). Identification of functionally important domains of human cytomegalovirus gO that act after trimer binding to receptors. PLoS Pathogens, 18(4), e1010452.

Publication 2022

Amino-Link Zeba buffer exchange columns

Agarose Antibodies Buffer Glycine Human Ovalbumin Peptides Saline Sds page Sera Tris

Corresponding Organization : Stanford University

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Variable analysis

independent variables

Ova-conjugated peptides 20, 26 or ovalbumin immobilized to agarose

dependent variables

Bound antibodies from pooled human sera

control variables

Pooled human sera that has been previously described [48 (link)]
Elution buffer (0.1M glycine-pH 2.2)
Neutralization buffer (0.1M Tris-pH 9.0)
Buffer exchange (Tris-Saline pH-7.2 using Zeba buffer exchange columns)
Antibody quantification method (SDS-PAGE and Coomassie staining)

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