Evaluating Dovitinib and KY-05009 Cytotoxicity

PBMCs or RPMI8226 cells (1.0×10⁴ cells/well) were seeded in 96-well plates and incubated for 24 h. After incubation, the cells were treated with dovitinib (Selleck Chemicals, USA) and/or KY-05009 [12 (link)] in complete medium containing 5% FBS for 24-72 h. Cell viability was measured using the Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) according to the manufacturer's instructions. The absorbance was measured using the Multiscan™ FC microplate photometer (Thermo Fisher Scientific, Boston, MA, USA). Experiments were performed in triplicate.

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Lee Y., Jung J.I., Park K.Y., Kim S.A, & Kim J. (2017). Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells. Oncotarget, 8(25), 41091-41101.

Publication 2017

dovitinib Cell Counting Kit-8 Multiscan™ FC microplate photometer

Cell viability Cells Dovitinib Ky 05009

Corresponding Organization :

Other organizations : Yonsei University, Eulji University

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Variable analysis

independent variables

Dovitinib (Selleck Chemicals, USA)
KY-05009 [12 (link)]

dependent variables

Cell viability

control variables

PBMCs or RPMI8226 cells (1.0×10^4 cells/well)
Complete medium containing 5% FBS
Incubation time (24-72 h)

controls

No controls explicitly mentioned.

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