Whole-mount PECAM1 staining was performed as previously described (Yang et al., 2020 (link)). In brief, hearts were dissected from mouse embryos in ice-cold PBS and fixed in 4% PFA at 4 °C overnight. Tissues were blocked in 0.2% Triton 100 solution containing 5% BSA and 2% horse serum and stained with anti-PECAM1 antibody (BD bioscience, 550274; 1:20) overnight at 4°C. Subsequently, the samples were washed and incubated with Alexa-conjugated secondary antibodies (Invitrogen) for 2 h at room temperature. The pictures were acquired with an Olympus IX73 fluorescence microscope.
Free full text: Click here
