Generation of PMVT-Derived hiPSC Lines

The hiPSC lines were generated from a blood sample of a 32-year-old female patient exhibiting the short-coupled PMVT at rest and carrying the RyR2-H29D mutation, as previously characterized [6 (link)] with the consent of the index patient and following the approval by the Institutional Review Board of Weill Cornell Medicine (NY, USA). As previously described, the RyR2-H29D mutation was corrected using CRISP/Cas9 technology thus generating an isogenic control hiPSC line (PMVT-29-corrected) [7 (link)]. The hiPSC lines were maintained on hES-qualified Matrigel (Corning, 354,277) at standard conditions (21% O₂, 5% CO₂ and 37 °C) and were enzymatically dissociated using TrypLE enzyme (Gibco, ref: 12,604–013) and passaged every 4 days. As 2 independent clones of PMVT RyR2-H29D hiPSC (C1, C3) tested in a previous study [7 (link)] exhibited identical properties, here we focused on the C1 clone only.