Evaluating Lipid and Kidney Function in PHL Rats

Plasma concentrations of total cholesterol, LDL cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, blood urea nitrogen (BUN), glutamate pyruvate transaminase (GPT), and glutamate oxaloacetate transaminase (GOT) were measured in CON and PHL rats (Green Cross Reference Lab, Seoul, Republic of Korea). To evaluate creatinine clearance (CL_CR), urine samples were collected for 24 h and urine volumes and creatinine concentrations were measured, with CL_CR estimated by dividing the amount of creatinine excreted in the urine over 24 h by the area under the plasma concentration–time curve of creatinine from 0 to 24 h (AUC_{0–24 h}) [26 (link)]. In addition, whole kidneys and livers were removed from CON and PHL rats and weighed, and biopsy samples were fixed in 10% neutral buffered formalin (BBC Biochemical, Mount Vernon, WA, USA).