Mouse prostate organoids from wild-type (WT), Pten−/− and Pten−/−;AmpkACT mice were generated and cultured as previously described.52 (link) However, collagenase/hyaluronidase (STEMCELL Technologies no. 07912) digestion at 37°C was reduced from 3 h to 2 h and E-cadherin PerCP-Efluor710 antibody (eBiosciences 46-3249-82) was substituted with E-cadherin PE antibody (Biolegend 14730) for sorting of prostate epithelial cells.
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