The experimental data of acylcarnitine (ten samples in each group) were expressed as the Mean ± standard error of Mean (`Mean ± SEM) and analyzed with SPSS Statistics 26.0 software, using Student-t test (two-side). GraphPad Prism5 software was used for statistical analysis and bar plot across all staining counts data. Differences between the two groups were considered to be significant when the p-value < 0.05. The relationship between differential metabolites and differentially expressed genes. Cytoscape v3.9.0 was used to plot the network diagram for the selected parts with a p-value < 0.001. The correlation analysis between DEGs and DEMs was evaluated by the cor function in the R package and the network plot was performed with Cytoscape v3.9.0. The analysis of metabolomics and transcriptomics data, which was performed with four independent samples in each group (transcriptomics control group only with three groups) were generated with MetaboAnalyst 5.0 and OmicStudio (https://www.omicstudio.cn ), showing with the PCA plots, volcano plots, advanced heatmap plots, and enrichment plots. The significant enriched biological pathways performed for DEMs and DEGs were screened with relative counts number, enrich factors and p-value of the selected enriched pathways. Information in detail about OmicStudio was shown elsewhere (44 (link), 45 (link)).
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