Differential Gene Expression Analysis

The microarrays used in this work have been already described [5 (link)]. RNA was extracted from the culture samples at exponential (22.5 h, 46.5 h) and stationary (60 h) phase, and analysis were performed for two biological replicates for each condition (two strains and three growth times). Labeling of RNA preparations with Cy3-dCTP, labeling of genomic DNA as the reference sample with Cy5-dCTP (2.5 pmol/50 μl hybridization solution), and the purification procedures were carried out as described previously [43 (link)]. The hybridization conditions, washing, scanning with Agilent Scanner G2565BA, and the quantification of the images were accomplished as previously described [44 (link)].