Production and Quantification of HCVcc

Production of cell culture‐derived infectious HCV (HCVcc) tagged with Gaussia luciferase from the Jc1FLAG2(p7-nsGluc2A) construct (genotype 2a, provided by Dr. Charles M. Rice) has been described elsewhere27 (link). HCV viral titer was expressed as focus forming units (FFU) and determined by immunofluorescence staining using primary anti-HCV NS5A mouse antibody (1:25,000; clone 9E10, gift from Dr. Charles M. Rice), followed by Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (H+L) (1:400; Invitrogen) as previously reported27 (link).

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Chung C.Y., Liu C.H., Wang G.H., Jassey A., Li C.L., Chen L., Yen M.H., Lin C.C, & Lin L.T. (2016). (4R,6S)-2-Dihydromenisdaurilide is a Butenolide that Efficiently Inhibits Hepatitis C Virus Entry. Scientific Reports, 6, 29969.

Publication 2016

Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (H+L)

Alexa fluor 488 Anti hcv antibody Anti immunoglobulin Cell culture Clone Genotype Goat Immunofluorescence Infectious Luciferase Mouse Rice

Corresponding Organization :

Other organizations : Kaohsiung Medical University, Taipei Medical University, Xiamen University

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Variable analysis

independent variables

Production of cell culture‐derived infectious HCV (HCVcc) tagged with Gaussia luciferase from the Jc1FLAG2(p7-nsGluc2A) construct (genotype 2a)

dependent variables

HCV viral titer expressed as focus forming units (FFU)

control variables

Primary anti-HCV NS5A mouse antibody (1:25,000; clone 9E10, gift from Dr. Charles M. Rice)
Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (H+L) (1:400; Invitrogen)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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