Plasma I-FABP Concentration Analysis

Plasma I-FABP concentrations were determined using EDTA plasma via enzyme-linked immunosorbent assay kits (HK406, Hycult Biotech Inc., Pennsylvania, USA). All analyses for each participant were completed within the same run for each measure. The intra-assay coefficient of variation was 4.4%. We planned to analyze absolute values. However, since there was a large inter-participant variability in plasma I-FABP in the present study as was the case in a previous study [21 (link)], we presented plasma I-FABP concentration as changes compared with fasting (0900) values.

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Tataka Y., Haramura M., Hamada Y., Ono M., Toyoda S., Yamada T., Hiratsu A., Suzuki K, & Miyashita M. (2022). Effects of oral cystine and glutamine on exercise-induced changes in gastrointestinal permeability and damage markers in young men. European Journal of Nutrition, 61(5), 2331-2339.

Publication 2022

HK406

Assay Edta Enzyme linked immunosorbent assay I fabp Plasma

Corresponding Organization : Waseda University

Other organizations : Ajinomoto (Japan)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma I-FABP concentrations

control variables

Plasma I-FABP concentrations were analyzed within the same run for each participant
Intra-assay coefficient of variation was 4.4%

controls

No positive or negative controls were explicitly mentioned in the provided information

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