Colorectal cancer (DLD-1) cells were transfected with plasmid DNA (pCI-neo and pCI-neo-p8). The day before transfection, DLD-1 cells were plated in 6-well plates at a density of 7 × 105 cells per well. After incubating overnight, cells were transfected using Lipofectamine 3000 (Invitrogen) in accordance with the manufacturer’s instructions [31 (link)]. The transfected cells were selected in RPMI 1640 containing antibiotics. (G-418) (Sigma, St. Louis, MO, USA).
Optimized P8 Gene Expression in Mammalian Cells
Colorectal cancer (DLD-1) cells were transfected with plasmid DNA (pCI-neo and pCI-neo-p8). The day before transfection, DLD-1 cells were plated in 6-well plates at a density of 7 × 105 cells per well. After incubating overnight, cells were transfected using Lipofectamine 3000 (Invitrogen) in accordance with the manufacturer’s instructions [31 (link)]. The transfected cells were selected in RPMI 1640 containing antibiotics. (G-418) (Sigma, St. Louis, MO, USA).
Corresponding Organization : Cell Biotech (South Korea)
Variable analysis
- Transfection of DLD-1 cells with plasmid DNA (pCI-neo and pCI-neo-p8)
- Transfected cells were selected in RPMI 1640 containing antibiotics (G-418)
- DLD-1 cells were plated in 6-well plates at a density of 7 × 10^5 cells per well before transfection
- Lipofectamine 3000 was used for transfection according to the manufacturer's instructions
- Positive control: pCI-neo plasmid
- Negative control: Not explicitly mentioned
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