Mouse bone marrow-derived mesenchymal stem cells (mMSC) were obtained from Life Technologies (Grand Island, NY) and grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 containing MSC-Qualified FBS (Gibco, Grand Island, NY). Human bone marrow-derived mesenchymal stem cells (hMSC) were obtained as previously described.21 (link)–24 (link) Non-malignant human hepatocytes (HH) were obtained from Sciencell (Carlsbad, CA) and cultured as recommended by the supplier.
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Isolation and Culture of MSCs and Hepatocytes
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Corresponding Organization : Mayo Clinic in Florida
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Variable analysis
independent variables
- Mesenchymal stem cell source (mouse bone marrow-derived vs. human bone marrow-derived)
- Unspecified
- Culture medium (DMEM/Nutrient Mixture F-12 containing MSC-Qualified FBS)
- Non-malignant human hepatocytes (HH) as a control cell type
- Positive control: Non-malignant human hepatocytes (HH)
- Negative control: Not explicitly mentioned
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