Antibiotic Cocktail for Gut Microbiome Modulation

Antibiotic treatment started with three days of amphotericin-B (Bristol Meyers Squibb, New York City, NY) 0.1 mg/ml, administered by gavage 12h⁻¹ (Figure 2A). From day three, water flasks were supplemented with ampicillin (Bristol Meyers Squibb, New York City, NY) 1 g/l and antibiotic concoction consisting of vancomycin (Abbot, Abbot Park, IL) 5 mg/ml, neomycin (Invitrogen, Carlsbad, CA) 10 mg/ml, metronidazol (Actavis, Hafnarfjordur, Iceland) 10 mg/ml, and amphotericin-B (Bristol Meyers Squibb) 0.1 mg/ml was administered by antibiotic gavage 12h⁻¹. Gavage volume of 10 ml/kg body weight was delivered with a stainless steel tube without prior sedation of the mice. Fresh antibiotic concoction was mixed every day and ampicillin and water was renewed every 7th day.