Identifying Nintedanib's Regulatory Nodes

To identify the nodes that regulate the effect of nintedanib on ECs and pericytes we performed a sensitivity analysis in the presence and absence of nintedanib. Knocking down each node in the absence of nintedanib describes the role of that node in nintedanib treatment. Comparing the difference in node activity in the untreated control compared to the activity in the presence of nintedanib describes the role of that node in regulating nintedanib’s effect on EC and pericyte cell fate. The nodes that have a differential effect dependent on nintedanib are identified as the sub-network that can be used as a mechanistic description of nintedanib activity. The sub-network analysis is performed in MATLAB then visualized used Cytoscape^{111 (link)}.