EMSA assays were done using 14% polyacrylamide (40% (w/v)29:1 Acrylamide:Bis-Acrylamide) gels in TBE buffer (Tris 0.13 M, Borate 45 mM, EDTA 2.5 mM pH 7.6 and run in TBE buffer at 4° at 160 V for 1 h, using Xylene cyanol as loading dye. Gels were stained with toluidine blue for 5 min, then rinsed with water overnight on a gel rotator.
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