Quantitative Analysis of Lung Collagen

Collagen content was analyzed using an optical microscope and image analyzer. Images were captured by a DFC 420 camera at 400 × magnification, which was attached to a Leica DM2500 trinocular optical microscope (Leica Microsystems, Wetzlar, Germany), and analyzed using Image-Pro Plus 4.5 software (NIH, Bethesda, MD, USA). To detect fibers, the optical density measurement method was used, and the software provided a threshold for positive areas and quantified them based on the determined area. Ten fields of alveolar septa per animal were examined. The results were expressed as a percentage of the positive area in comparison to the total area^{27 (link),28 (link)}.

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Galli T.T., de Campos E.C., do Nascimento Camargo L., Fukuzaki S., dos Santos T.M., Hamaguchi S.S., Bezerra S.K., Silva F.J., Rezende B.G., dos Santos Lopes F.T., Olivo C.R., Saraiva-Romanholo B.M., Prado C.M., Leick E.A., Bourotte C.L., Benseñor I.J., Lotufo P.A., Righetti R.F, & Tibério I.F. (2024). Effects of environmental exposure to iron powder on healthy and elastase-exposed mice. Scientific Reports, 14, 9134.

Publication 2024

DFC 420

Corresponding Organization : Universidade de São Paulo

Other organizations : Fundação Faculdade de Medicina, Universidade Federal de São Paulo, Hospital Sírio-Libanês

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Collagen content

control variables

Magnification of the optical microscope (400×)
Microscope model (Leica DM2500 trinocular optical microscope)
Camera model (DFC 420 camera)
Image analysis software (Image-Pro Plus 4.5)
Number of fields examined per animal (10 fields of alveolar septa)

controls

None explicitly mentioned

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