Quantitative Western Blot Analysis

Western blot was performed as previously described [40 (link)]. The antibodies used were listed as below: DOT1L (ab64077, Abcam), CDK2 (2546, CST), Cyclin A2 (4656, CST), PCNA (13110, CST), GAPDH (51332, CST), c-Myc (5605, CST), H3K79me1 (ab2886, Abcam, Cambridge, MA, USA), H3K79me2 (ab3594, Abcam), H3K79me3 (ab2621, Abcam), and H3 (17168-1-AP, Proteintech, Wuhan, China). Gray ratio of each blot was analyzed by using the Image J ver. 1.46 software and protein/GAPDH or protein/H3 ratio was shown.

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Yang L., Lei Q., Li L., Yang J., Dong Z, & Cui H. (2019). Silencing or inhibition of H3K79 methyltransferase DOT1L induces cell cycle arrest by epigenetically modulating c-Myc expression in colorectal cancer. Clinical Epigenetics, 11, 199.

Publication 2019

ab64077 ab2886 ab3594 ab2621

Antibodies C myc Cdk2 Cyclin a2 Dot1l Gapdh Pcna Protein Western blot

Corresponding Organization : Southwest University

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Variable analysis

independent variables

Antibodies used: DOT1L, CDK2, Cyclin A2, PCNA, GAPDH, c-Myc, H3K79me1, H3K79me2, H3K79me3, H3

dependent variables

Gray ratio of each blot analyzed using Image J ver. 1.46 software
Protein/GAPDH ratio
Protein/H3 ratio

control variables

Western blot procedure was performed as previously described [40]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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