Minocycline Ocular Uptake Dynamics

The intrinsic fluorescence intensity of minocycline was observed using a fluorescence microscope, with cell nuclei being labeled with DAPI. MINO@PLGA and minocycline were smeared on slides and calibrated under a fluorescence microscope. Rats were then grouped by various time points and administered the drug once. At the designated time points, rat eyeballs and surrounding tissues were harvested and processed into frozen sections. The frozen sections were fixed in cold acetone for 10 min, then washed, and subsequently mounted using a DAPI medium. Fluorescent images of the cornea were captured using a confocal microscope, employing the specified parameters.