Antioxidant Capacity Evaluation Techniques

Total phenolic content (TPC) was measured using 0.2 N Folin–Ciocalteu reagent (Sigma, St Louis, MO, USA) according to Lu et al. [18 (link)]. Ferric Reducing/Antioxidant Power (FRAP) was tested by a working reagent (mixture of 300 µM acetate buffer, 10 mM TPTZ solution and 20 mM FeCl₃ at 10:1:1 (v/v/v)) based on the method from Rachman, A. Brennan, Morton and Brennan [2 (link)]. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Radical Scavenging Capacity was determined following Rachman, A. Brennan, Morton and Brennan [2 (link)] using ABTS working solution.

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Wang J., Brennan M.A., Brennan C.S, & Serventi L. (2022). Predictive Glycaemic Response of Pasta Enriched with Juice, Puree, and Pomace from Red Cabbage and Spinach. Nutrients, 14(21), 4575.

Publication 2022

0.2 N Folin–Ciocalteu reagent

Abts Acetate Antioxidant Buffer Folin Sulfonic acid

Corresponding Organization : Lincoln University

Other organizations : Riddet Institute, RMIT University

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Variable analysis

independent variables

Total phenolic content (TPC) measurement using 0.2 N Folin–Ciocalteu reagent
Ferric Reducing/Antioxidant Power (FRAP) test using a working reagent (mixture of 300 µM acetate buffer, 10 mM TPTZ solution and 20 mM FeCl3 at 10:1:1 (v/v/v))
ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Radical Scavenging Capacity determination

dependent variables

Total phenolic content (TPC)
Ferric Reducing/Antioxidant Power (FRAP)
ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Radical Scavenging Capacity

control variables

Folin–Ciocalteu reagent (Sigma, St Louis, MO, USA)
TPTZ solution
FeCl3
ABTS working solution

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