Total phenolic content (TPC) was measured using 0.2 N Folin–Ciocalteu reagent (Sigma, St Louis, MO, USA) according to Lu et al. [18 (link)]. Ferric Reducing/Antioxidant Power (FRAP) was tested by a working reagent (mixture of 300 µM acetate buffer, 10 mM TPTZ solution and 20 mM FeCl3 at 10:1:1 (v/v/v)) based on the method from Rachman, A. Brennan, Morton and Brennan [2 (link)]. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Radical Scavenging Capacity was determined following Rachman, A. Brennan, Morton and Brennan [2 (link)] using ABTS working solution.
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