Cell lysis, the measurement of lysate protein concentration, and Western blotting were conducted as previously described [21 (link)]. Primary Antibody Dilution Buffer (P0023A, Beyotime) was used to dilute primary antibodies, including NEIL3 (GTX55960, GeneTex, 1:1000), β-actin (60008-1-Ig, Proteintech, 1:5000), and GAPDH (D16H11, CST, 1:1000).
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