The following plasmids were previously described: pGa981/6, pcDNA3-Flag-SPOC, pcDNA3-RBP;33 (link) pcDNA3-Flag-SPOC pcDNA3-F3, pcDNA3-F3-AML1-ETO, pcDNA3-AML1-ETO(tr)-GFP;31 (link) pcDNA3-Flag-SPOC (Y3602A), pcDNA3-Flag-SPOC (K3516A), pcDNA3-Flag-SPOC (R3552A/R3554A), pcDNA3-RBP-SPOC pcDNA3-RBP-SPOC (Y3602A), pcDNA3-RBP-SPOC (K3516A), pcDNA3-RBP-SPOC (R3552A/R3554A).34 (link) The plasmid pMYs-IRES-GFP was commercially achieved from Cell Biolabs, San Diego, CA, USA. Sequence information of all additional vectors used in this study is available on request.
Protocol detail
Plasmid Characterization and Cloning
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Aml1
Cell
Ires
Plasmid
Vectors
Corresponding Organization : Cincinnati Children's Hospital Medical Center
Other organizations : Universität Ulm, University of Giessen
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Variable analysis
independent variables
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- Not explicitly mentioned
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