Samples were mounted in PBS, and images of eggs, embryos, and beads were obtained with a confocal microscope (LSM 510; Carl Zeiss) using a 63×/1.2 NA water immersion objective lens at room temperature (Baibakov et al., 2007 (link); Yauger et al., 2011 (link)). LSM 510 images were exported as full-resolution TIF files and processed in Photoshop CS5.5 (Adobe) to adjust brightness and contrast. Alternatively, confocal optical sections were projected to a single plane with maximum intensity and combined with DIC images of eggs or peptide beads using LSM image software.
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Confocal Imaging of Eggs, Embryos, and Beads
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Corresponding Organization : National Institutes of Health
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Variable analysis
independent variables
- Mounting samples in PBS
- Images of eggs, embryos, and beads
- Use of a confocal microscope (LSM 510; Carl Zeiss)
- Use of a 63×/1.2 NA water immersion objective lens
- Experiments conducted at room temperature
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